Nā mea hoʻoheheʻe ʻana i ka ʻakika Nucleic Gargle
KUMU HOIKE
ʻO ka huahana ka nui o ka gargle, superparamagnetic nanospheres hui pū ʻia, a me nā reagents lysis kūikawā.Loaʻa ka pilina maikaʻi no nā ʻāpana kino (me nā maʻi maʻi manuahi a me nā pūnaewele i hoʻopili ʻia).I ka hoʻopili ʻana i ka solution lysis, hiki i ka mea non-ionic cell/nucleus-membrane-breaking surfactants a me ka protease inhibitors i loko o ka hopena ke kāohi i ka hana o ka DNA/RNA enzyme, a hoʻopaʻa i ka waikawa nucleic.Hoʻokuʻu maikaʻi ʻia nā mea wai nucleic a pau i loko o nā ʻāpana kino o ka gargle i loko o ka hopena lysis, e loaʻa koke i ka waika nucleic.ʻAʻole pono ka hoʻomaʻemaʻe ʻana i ka waika nucleic i nā laʻana gargle e hoʻohana ana i kēia pahu, hiki ke hoʻopili pololei i ka pahu ʻike nucleic acid i lalo.
Nā mea nui o ka reagent
Hōʻike ʻia nā ʻāpana ma ka papa 1.
Papa 1 Nā ʻāpana a me ka hoʻouka ʻana i ka Kit
| inoa mea | Nā mea nui | Nui (1) | Nui (10) | Nui (30) | Nui (50) |
| 1. Kāmau A | NaCl | 8mL/puu | 8mL/puu *10 paipu | 8mL/pu *30 paipu | 8mL/pu *50 paipu |
| 2. ʻOhi ʻOhi | PP | 1 apana | 10 pcs | 30 pcs | 50 pcs |
| 3. Haʻina Hoʻonui B | Nā lei mākēneki | 2mL/puu | 2mL/pu *10 paipu | 2mL/pu *30 paipu | 2mL/pu *50 paipu |
| 4 ʻO Lysis Buffer C | ʻO Protease K | 0.2mL/ ʻāpana | 0.2mL / ʻāpana * 10 pcs | 0.2mL / ʻāpana * 30 pcs | 0.2mL / ʻāpana * 50 pcs |
| 5. Kāpena Magnetic | Mākēneki | 1 apana | 10 pcs | 30 pcs | 50 pcs |
Pono nā ʻāpana no 12 mahina.
ʻO nā ʻāpana i koi ʻia i ka unuhi ʻana o ka nucleic acid, akā ʻaʻole i hoʻokomo ʻia i loko o ka pahu:
1. Mea hoopau: 1.5ml EP paipu;
2. Mea lako: ʻauʻau wai (a i ʻole ʻauʻau metala), pipettes, a centrifuge.
ʻIke kumu
Nā koi laʻana:
1. E pili ana i ka nucleic acid extraction a me ka hoʻonui ʻana i nā laʻana gargle.
2. E hoʻohui ʻia ka hāpana gargle i ka hopena hoʻonui B i ka manawa ma hope o ka hōʻiliʻili ʻana.E hoʻoneʻe koke ʻia nā peʻa magnetic i kiʻi ʻia i ka lysis buffer C.Hiki ke mālama ʻia nā laʻana i hoʻohui ʻia i ka lysis buffer C ma ka lumi wela.
Hōʻike kikoʻī: 1 ʻāpana / pahu, 10 pcs / pahu, 30 pcs / pahu, a me 50 pcs / pahu.
Nā kūlana mālama: Pono e mālama ʻia ka solution enrichment B a me lysis solution C no 12 mahina ma 2-8 ℃, a hiki ke mālama ʻia nā mea ʻē aʻe no 12 mahina ma lalo o ka mahana o ka lumi;Hiki ke lawe ʻia a mālama ʻia ka pahu ma lalo o ka mahana wela, ʻaʻole e ʻoi aku ma mua o 5 mau lā.
Manawa o ka mana: 12 mahina
No./ke koi ʻenehana huahana No.:HJXB Helu 20220086.
Lā o ka ʻae ʻia a me ka hoʻoponopono ʻana i nā ʻōlelo kuhikuhi:
Lā o ka ʻae ʻia: ʻOkakopa 26, 2022
