TAGMe DNA Methylation Detection Kits (qPCR) no ka maʻi maʻi ʻaʻai
NA HI'O HUA
pololei
Hoʻopaʻa ʻia ma luna o 3500 mau lāʻau lapaʻau i loko o nā haʻawina ʻelua makapō multi-center, ʻo ka huahana kahi kikoʻī o 92.7% a me kahi sensitivity o 82.1%.
ʻoluʻolu
Hiki ke hoʻopau ʻia ka ʻenehana ʻike ʻike methylation Me-qPCR kumu i hoʻokahi ʻanuʻu i loko o 3 mau hola me ka ʻole o ka hoʻololi bisulfite.
ʻAʻole hoʻokomo
ʻO 30 mL wale nō o ka laʻana mimi ke koi ʻia e ʻike i 3 ʻano o ka maʻi kanesa, me ka maʻi ʻaʻai pelvis renal, ka maʻi ʻaʻai ureteral, ka maʻi maʻi ʻaʻai i ka manawa like.
Nā hiʻohiʻona noiʻi
ʻIke kōkua
ʻO ka poʻe e loaʻa ana i ka hematuria ʻeha ʻole/ i manaʻo ʻia he urothelial (kani ʻaʻai ureteral/ maʻi ʻaʻai pelvis renal)
Ka Loiloi Pilikia
ʻO ka ʻokiʻoki a me ka chemotherapy-koi i ka heluna kanaka me ka carcinoma urothelial;
Nānā Hoʻi hou
ʻO ka heluna kanaka postoperative me ka carcinoma urothelial
KA MANAO ANA
Hoʻohana ʻia kēia kit no ka ʻike in vitro qualitative o ka hypermethylation o ka Urothelial Carcinoma (UC) gene i loko o nā specimens urothelial.Hōʻike ka hopena maikaʻi i ka piʻi ʻana o ka UC, kahi e koi ai i ka cystoscope a/a i ʻole ka nānā ʻana i ka histopathological.ʻO ka mea ʻē aʻe, hōʻike nā hopena hōʻike maikaʻi ʻole i ka haʻahaʻa o ka UC, akā ʻaʻole hiki ke hoʻopau loa i ka pilikia.Pono e hoʻokumu ʻia ka ʻike hope ma ka cystoscope a/a i nā hopena histopathological.
KUMU HOIKE
Loaʻa i kēia pahu ka mea hoʻoheheʻe nucleic acid a me ka reagent ʻike PCR.Hoʻokuʻu ʻia ka ʻakika nucleic e ka magnetic-bead-based method.Hoʻokumu ʻia kēia pahu ma luna o ke kumu o ka fluorescence quantitative PCR, me ka hoʻohana ʻana i ka methylation-specific real-time PCR reaction no ka nānā ʻana i ka DNA template, a ʻike pū i nā pūnaewele CpG o ka UC gene a me ka māka hoʻomalu maikaʻi i nā ʻāpana gene reference G1 a me G2.Ua helu ʻia ka pae methylation o UC gene, i kapa ʻia ʻo ka waiwai Me, e like me ka UC gene methylated DNA amplification Ct waiwai a me ka waiwai Ct o ka kuhikuhi.Hoʻoholo ʻia ka UC gene hypermethylation kūlana maikaʻi a maikaʻi ʻole paha e like me ka waiwai Me.
DNA Methylation Detection Kits (qPCR) no ka Urothelial Cancer
| Hoʻohana lapaʻau | ʻO ka hōʻailona kōkua hōʻailona o ka cnacer urothelial;ka loiloi ʻana i ka maikaʻi o ka hoʻomaʻamaʻa ʻana i ka ʻokiʻoki/chemothery;ka nānā ʻana i ka hoʻihoʻi ʻana ma hope o ka hana |
| ʻAno ʻike | UC |
| ʻAno laʻana | He mea hoʻoheheʻe ʻia ka mimi (sediment mimi) |
| ʻano hoʻāʻo | ʻO ka ʻenehana PCR nui fluorescence |
| Nā hiʻohiʻona kūpono | ABI7500 |
| Hoʻopili kikoʻī | 48 hōʻike / pahu |
| Nā Kūlana Waihona | Pono e mālama ʻia ʻo Kit A ma 2-30 ℃ Pono e mālama ʻia ʻo Kit B ma -20±5 ℃ Pono a hiki i 12 mahina. |
