TAGMe DNA Methylation Detection Kits (qPCR) no ka maʻi maʻi maʻi endometrial
NA HI'O HUA
pololei
Hoʻopaʻa ʻia ma luna o 800 mau lāʻau lapaʻau i loko o nā haʻawina ʻelua makapō multi-center, ʻo ka huahana kahi kikoʻī o 82.81% a me kahi sensitivity o 80.65%.
ʻoluʻolu
Hiki ke hoʻopau ʻia ka ʻenehana ʻike ʻike methylation Me-qPCR kumu i hoʻokahi ʻanuʻu i loko o 3 mau hola me ka ʻole o ka hoʻololi bisulfite.
Mamua
Hiki ke ʻike ʻia ma ka pae precancerous.
ʻOtometi
Hoʻohana ʻia me ka pulupulu cervical a me nā laʻana Pap smear.
KA MANAO ANA
Hoʻohana ʻia kēia kit no ka ʻike in vitro qualitative o ka hypermethylation o ka gene PCDHGB7 incervical specimens.ʻO ka hopena maikaʻi e hōʻike ana i ka piʻi ʻana o ka hopena o ka maʻi maʻi precancerous endometrial a me ka maʻi kanesa, kahi e pono ai ka nānā ʻana i ka histopathological o ka endometrium.ʻO ka mea ʻē aʻe, hōʻike nā hopena hōʻike maikaʻi ʻole i ka haʻahaʻa o ka hopena o ka endometrial precancerous lesions a me ka maʻi kanesa, akā ʻaʻole hiki ke kāpae ʻia ka pilikia.Pono e hoʻopaʻa ʻia ka ʻike hope loa ma nā hopena hoʻokolokolo histopathological o ka endometrium.ʻO PCDHGB7 kahi lālā o ka ʻohana protocadherin γ gene cluster.Ua ʻike ʻia ʻo Protocadherin e hoʻoponopono i nā kaʻina biological e like me ka hoʻonui ʻana o ka cell, cell cycle, apoptosis, invasion, migration and autophagy of tumor cells ma o nā ala hōʻailona like ʻole, a me kona silencing gene i kumu ʻia e ka hypermethylation o ka mea hoʻolaha e pili kokoke ana i ka hanana a me ka ulu ʻana. o na ma'i ma'i he nui.Ua hōʻike ʻia e pili ana ka hypermethylation o PCDHGB7 me nā ʻano ʻokoʻa like ʻole, e like me ka lymphoma non-Hodgkin, ka maʻi ʻaʻai umauma, ka maʻi maʻi cervical, ka maʻi maʻi endometrial a me ka maʻi maʻi ʻaʻai.
KUMU HOIKE
Loaʻa i kēia pahu ka mea hoʻoheheʻe nucleic acid a me ka reagent ʻike PCR.Hoʻokuʻu ʻia ka ʻakika nucleic e ka magnetic-bead-based method.Hoʻokumu ʻia kēia pahu ma ke kumu o ka fluorescence quantitative PCR, me ka hoʻohana ʻana i ka methylation-specific real-time PCR reaction no ka nānā ʻana i ka DNA template, a ʻike pū i nā pūnaewele CpG o PCDHGB7 gene a me ka marker control marker internal reference gene fragments G1 a me G2.Ua helu ʻia ka pae methylation o PCDHGB7 i ka laʻana, a i ʻole ka waiwai Me, e like me ka PCDHGB7 gene methylated DNA amplification Ct waiwai a me ka waiwai Ct o ka kuhikuhi.Hoʻoholo ʻia ka PCDHGB7 gene hypermethylation kūlana maikaʻi a maikaʻi ʻole paha e like me ka waiwai Me.
Nā hiʻohiʻona noiʻi
ʻO ka nānā mua
Nā kānaka olakino
Ka Loiloi Pilikia
ʻO nā pūʻulu pilikia nui (nā poʻe me ke kahe koko ʻokoʻa ma hope o ka menopause, ka mānoanoa endometrial, etc.)
Nānā Hoʻi hou
Ka heluna kanaka wānana
ʻO ke koʻikoʻi o ke kino
Ka nānā mua ʻana no ka heluna kanaka olakino:Hiki ke nānā pono ʻia ka maʻi maʻi endometrial a me nā ʻeha precancerous;
Ka loiloi pilikia no ka heluna kanaka pilikia nui:Hiki ke hoʻokō ʻia ka loiloi pilikia no ka poʻe me ke koko ʻokoʻa ʻole a me ka mānoanoa endometrial ma hope o ka menopause e kōkua i ka maʻi maʻi;
Ka nānā 'ana i ka heluna kanaka prognostic:Hiki ke hana ʻia ka nānā ʻana i ka heluna kanaka ma hope o ka hana ʻana no ka pale ʻana i ka lohi o ka mālama ʻana ma muli o ka hoʻi hou ʻana.
ʻOhi laʻana
ʻano laʻana: E kau i ka mea hoʻoheheʻe cervical ma ka cervical os, e hamo mālie i ka pulupulu cervical a hoʻololi i 4-5 mau manawa ma ka ʻākau, wehe mālie i ka pulupulu cervical, hoʻokomo i loko o ka solution mālama cell, a e lepili iā ia no ka nānā ʻana.
Mālama ʻia nā laʻana:Hiki ke mālama ʻia nā laʻana ma ka lumi wela a hiki i 14 lā, ma 2-8 ℃ a hiki i 2 mahina, a ma -20±5 ℃ a hiki i 24 mahina.
Kaʻina ʻike: 3 mau hola (me ka ʻole o ka hana lima)
TAGMe DNA Methylation Detection Kits (qPCR) no ka maʻi maʻi maʻi endometrial
| Hoʻohana lapaʻau | ʻO ka hōʻailona kōkua hōʻailona o ka carcinoma endometrial |
| ʻAno ʻike | PCDHGB7 |
| ʻAno laʻana | Nā hōʻailona cervical wahine |
| ʻano hoʻāʻo | ʻO ka ʻenehana PCR nui fluorescence |
| Nā hiʻohiʻona kūpono | ABI7500 |
| Hoʻopili kikoʻī | 48 hōʻike / pahu |
| Nā Kūlana Waihona | Pono e mālama ʻia ʻo Kit A ma 2-30 ℃ Pono e mālama ʻia ʻo Kit B ma -20±5 ℃ Pono a hiki i 12 mahina. |
E pili ana iā mākou
Loaʻa i ka Epiprobe kahi hana hoʻomohala holoʻokoʻa: uhi ʻia ke kikowaena hana GMP i kahi ʻāpana o 2200 square meters, a mālama i kahi ʻōnaehana hoʻokele maikaʻi ISO13485, e hoʻokō i nā koi hana o nā ʻano huahana reagent hoʻāʻo genetic;uhi ka hale hana lapaʻau i kahi ʻāpana o 5400 square meters a loaʻa iā ia ka hiki ke hoʻokō i ka ʻoihana ʻike methylation cancer ma ke ʻano he keʻena lāʻau lapaʻau ʻekolu ʻaoʻao.Ma waho aʻe, loaʻa iā mākou ʻekolu mau huahana i loaʻa ka palapala CE, e uhi ana i ka maʻi maʻi cervical, ka maʻi maʻi endometrial a me ka ʻike pili pili i ka maʻi urothelial.
Hiki ke ho'ohana 'ia ka 'ike loea ma'i 'a'ai 'o Epiprobe no ka nānā 'ana i ka ma'i 'a'ai mua, ka 'ikepili kōkua, ka loiloi mua a me ka postoperative, ka nānā 'ana i ka ma'i 'a'ai, e holo ana ma ke ka'ina holo'oko'a o ka ma'i 'a'ai ma'i 'a'ai a me ka lapa'au 'ana, e hā'awi ana i nā ha'ina maika'i no nā kauka a me nā ma'i.
