Mea Hoʻoemi Nucleic Acid (A01)
Kuʻuna ʻike
Ma hope o ka hoʻokuʻu ʻana i ka DNA genomic ma o ka hoʻokaʻawale ʻana i nā cell me ka lysis buffer, hiki i ka bead magnetic ke hoʻopaʻa koho i ka DNA genomic i ka hāpana.Hiki ke hoʻoneʻe ʻia kekahi helu liʻiliʻi o nā haumia i hoʻopaʻa ʻia e ka peʻa magnetic e ka mea holoi holoi.I loko o TE, hiki ke hoʻokuʻu i ka boundgenome DNA, e loaʻa ana ka DNA genome kiʻekiʻe.He maʻalahi a wikiwiki kēia ʻano a he kiʻekiʻe ka maikaʻi o ka DNA i unuhi ʻia, hiki ke hoʻokō i ke koi no ka ʻike ʻana i ka DNA methylation.Eia nō naʻe, hiki ke hoʻohālikelike ʻia ka pahu hoʻoheheʻe i hoʻokumu ʻia i ka peʻa magnetic me ka unuhi ʻana o ka nucleic acid, e hālāwai me nā hana hoʻoneʻe nucleic acid kiʻekiʻe.
Nā mea nui o ka reagent
Hōʻike ʻia nā ʻāpana i ka papa 1:
Papa 1 Reagent Companies and Loading
| inoa mea | Nā mea nui | Nui (48) | Nui (200) |
| 1. Hoʻonā Lysis | Guanidine hydrochloride, Tris | 11 mL/'ōmole | 44 mL/'ōmole |
| 2. Hoʻomaʻemaʻe hoʻomaʻemaʻe A | NaCl, Tris | 11 mL/'ōmole | 44 mL/'ōmole |
| 3. Nā hāʻina hoʻomaʻemaʻe B | NaCl, Tris | 13 mL/'ōmole | 26.5mL/'ōmole *2 |
| 4. Eluent | Tris, EDTA | 12 mL/'ōmole | 44 mL/'ōmole |
| 5. Hoʻonā K | ʻO Protease K | 1.1mL / ʻāpana | 4.4mL / ʻāpana |
| 6. Hoʻokuʻu ʻia ʻo Magnetic bead 1 | Nā lei mākēneki | 1.1mL / ʻāpana | 4.4mL / ʻāpana |
| 7. Nā ʻōlelo aʻoaʻo no ka unuhi ʻana i nā mea hoʻokalakupua nucleic acid |
| 1 kope | 1 kope |
ʻO nā ʻāpana i koi ʻia i ka unuhi ʻana o ka nucleic acid, akā ʻaʻole i hoʻokomo ʻia i loko o ka pahu:
1. Reagent: Anhydrous ethanol, isopropanol a me PBS;
2. Nā mea hoʻohana: 50ml centrifuge tube and1.5ml EP tube;
3. Lako: Kettle ʻauʻau wai, pipettor, papa magnetic, centrifuge, 96-hole pā hohonu (akomi), ʻakomi nucleic acid extraction lako (akomi).
ʻIke kumu
Nā koi laʻana
1. Pono e 'alo 'ia ka ho'opa'a 'ia ma waena o nā la'ana ma ka hō'ili'ili a me ka waiho 'ana.
2. E hoʻopau ʻia ka ʻike ma lalo o ka mālama ʻana i nā lā 7 o ka mahana ambient ma hope o ka hōʻiliʻili ʻana o ka cervical exfoliated cell sample (non-fixed).E hoʻopau ʻia ka ʻike ma lalo o ka mālama ʻana i nā lā he 30 o ka mahana ambient ma hope o ka hōʻiliʻili ʻana i ke ʻano mimi;E hoʻopau ʻia ka ʻike ʻana i ka manawa ma hope o ka hōʻiliʻili ʻana i nā laʻana cell moʻomeheu.
Hōʻike kaʻa:200 pcs / pahu, 48 pcs / pahu.
Nā kūlana mālama:2-30 ℃
Manawa o ka mana:12 mahina
Mea pili:Tianlong NP968-C nucleic acid extraction instrument, Tiangen TGuide S96 nucleic acid extraction instrument, GENE DIAN EB-1000 nucleic acid extraction instrument.
No./ke koi ʻenehana huahana No.: HJXB Helu 20210099.
Lā o ka ʻae ʻia a me ka hoʻololi ʻana i nā ʻōlelo kuhikuhi:
La aponoia: Nov. 18, 2021
