Mea Hoʻoemi Nucleic Acid (A02)
Kuʻuna ʻike
Ma hope o ka hoʻokuʻu ʻana i ka DNA genomic ma o ka hoʻokaʻawale ʻana i nā cell me ka lysis buffer, hiki i ka bead magnetic ke hoʻopaʻa koho i ka DNA genomic i ka hāpana.Hiki ke hoʻoneʻe ʻia kekahi helu liʻiliʻi o nā haumia i hoʻopaʻa ʻia e ka peʻa magnetic e ka mea holoi holoi.I loko o TE, hiki ke hoʻokuʻu i ka boundgenome DNA, e loaʻa ana ka DNA genome kiʻekiʻe.He maʻalahi a wikiwiki kēia ʻano a he kiʻekiʻe ka maikaʻi o ka DNA i unuhi ʻia, hiki ke hoʻokō i ke koi no ka ʻike ʻana i ka DNA methylation.Eia nō naʻe, hiki ke hoʻohālikelike ʻia ka pahu hoʻoheheʻe i hoʻokumu ʻia i ka peʻa magnetic me ka unuhi ʻana o ka nucleic acid, e hālāwai me nā hana hoʻoneʻe nucleic acid kiʻekiʻe.
Nā mea nui o ka reagent
Hōʻike ʻia nā ʻāpana ma ka papa 1:
Papa 1 Reagent Companies and Loading
| inoa mea | Nā mea nui | Nui (48) | Nui (200) |
| 1. Hoʻopaʻa hoʻoheheʻe ʻana A | Tris, SDS | 15.8 mL/'ōmole | 66mL/'ōmole |
| 2. Lysis Buffer L | Guanidinium Isothiocyanate, Tris | 15.8 mL/'ōmole | 66mL/'ōmole |
| 3. Holoi Pahu A | NaCl, Tris | 11 mL/'ōmole | 44 mL/'ōmole |
| 4. Holoi Pahu B | NaCl, Tris | 13 mL/'ōmole | 26.5mL/'ōmole *2 |
| 5. TE | Tris, EDTA | 12 mL/'ōmole | 44 mL/'ōmole |
| 6. Hoʻonā K | ʻO Protease K | 1.1mL / ʻāpana | 4.4mL / ʻāpana |
| 7. Hoʻokuʻu ʻia ʻo Magnetic bead 2 | Nā lei mākēneki | 0.5mL / ʻāpana | 2.2mL / ʻāpana |
| 8. Nā ʻōlelo aʻoaʻo no ka unuhi ʻana i nā mea hoʻokalakupua nucleic acid | / | 1 kope | 1 kope |
ʻO nā ʻāpana i koi ʻia i ka unuhi ʻana o ka nucleic acid, akā ʻaʻole i hoʻokomo ʻia i loko o ka pahu:
1. Reagent: Anhydrous ethanol, isopropanol, a me PBS;
2. Nā mea hoʻohana: 50mL centrifuge tube and1.5mL EP tube;
3. Mea lako: ʻauʻau wai, pipettes , papa magnetic, centrifuge, 96-well plate (akomi), ʻakomi nucleic acid extraction lako (akomi).
ʻIke kumu
Nā koi laʻana:
1. E hoʻopau ʻia ka ʻike ma lalo o ka mālama ʻana i nā lā 7 o ka mahana ambient ma hope o ka hōʻiliʻili ʻana o ka cervical exfoliated cell sample (non-fixed).
2. E hoʻopau ʻia ka ʻike ma lalo o ka mālama ʻana i nā lā 30 o ka mahana ambient ma hope o ka hōʻiliʻili ʻana o ka cervical exfoliated cell sample (paʻa).
3. E hoʻopauʻia kaʻikeʻana ma lalo o ka mālamaʻana i nā lā he 30 o ka mahana wela ma hope o ka hōʻiliʻiliʻana i ka hōʻailona mimi;E hoʻopau ʻia ka ʻike ʻana i ka manawa ma hope o ka hōʻiliʻili ʻana i nā laʻana cell moʻomeheu.
Hōʻike kaʻa:200 pcs / pahu, 48 pcs / pahu.
Nā kūlana mālama:2-30 ℃
Manawa o ka mana:12 mahina
Mea pili:Tianlong NP968-C nucleic acid extraction instrument, Tiangen TGuide S96 nucleic acid extraction instrument, GENE DIAN EB-1000 nucleic acid extraction instrument.
No./ke koi ʻenehana huahana No.:HJXB Helu 20210100.
Lā o ka ʻae ʻia a me ka hoʻoponopono ʻana i nā ʻōlelo kuhikuhi:La aponoia: Nov. 18, 2021
E pili ana iā mākou
Ma ke ʻano he ʻoihana ʻenehana kiʻekiʻe i hoʻokumu ʻia ma 2018 e nā loea epigenetic kiʻekiʻe, ʻo Epiprobe ka nānā ʻana i ka maʻi molecular o ka maʻi maʻi DNA methylation a me ka ʻoihana theranostics precision.Me ke kumu ʻenehana hohonu, manaʻo mākou e alakaʻi i ke au o nā huahana hou e hoʻopau i ka maʻi kanesa i ka ʻōpuʻu!
Ma muli o ka noiʻi lōʻihi o ka hui Epiprobe core, hoʻomohala a me ka hoʻololi ʻana i ke kahua o ka methylation DNA me nā mea hou o ka ʻoki ʻana, i hui pū ʻia me nā pahuhopu DNA methylation kūʻokoʻa o nā maʻi maʻi maʻi, hoʻohana mākou i kahi algorithm multivariate kū hoʻokahi e hui pū ana i ka ʻikepili nui a me ka ʻenehana naʻauao hana. hoʻomohala kūʻokoʻa i kahi ʻenehana biopsy wai pale patent kūʻokoʻa.Ma ka nānā ʻana i ka pae methylation o nā wahi kikoʻī o nā ʻāpana DNA manuahi i loko o ka laʻana, ua pale ʻia nā hemahema o nā ʻano loiloi kuʻuna a me nā palena o ka hōʻoki ʻana a me ka hōʻiliʻili ʻana i ka puncture, ʻaʻole ia e loaʻa i ka ʻike pololei ʻana i nā maʻi maʻi maʻi mua, akā hiki ke nānā i ka manawa maoli. o ka ma'i 'a'ai 'ana a me ka ulu 'ana.
