TAGMe DNA Methylation Detection Kits (qPCR) no ka maʻi maʻi maʻi ʻāʻī / maʻi maʻi endometrial
NA HI'O HUA
ʻAʻole hoʻokomo
Hoʻohana ʻia me ka pulupulu cervical a me nā laʻana Pap smear.
ʻoluʻolu
Hiki ke hoʻopau ʻia ka ʻenehana ʻike ʻike methylation Me-qPCR kumu i hoʻokahi ʻanuʻu i loko o 3 mau hola me ka ʻole o ka hoʻololi bisulfite.
Mamua
Hiki ke ʻike ʻia ma ka pae precancerous.
ʻOtometi
Hoʻopili ʻia me ka polokalamu loiloi hopena maʻamau, ʻokoʻa ka wehewehe ʻana i nā hopena a hiki ke heluhelu pololei ʻia.
Nā hiʻohiʻona noiʻi
ʻO ka nānā mua
Nā kānaka olakino
Ka Loiloi Pilikia
Ka heluna kanaka pilikia nui (maikaʻi no ka papillomavirus kanaka kiʻekiʻe (hrHPV) a maikaʻi paha no ka cytology exfoliation cervical / maikaʻi no ka papillomavirus kanaka kiʻekiʻe (hrHPV) a i ʻole maikaʻi no ka cytology exfoliation cervical)
Nānā Hoʻi hou
Ka heluna kanaka wānana
KA MANAO ANA
Hoʻohana ʻia kēia kit no ka ʻike in vitro qualitative o ka hypermethylation o ka gene PCDHGB7 i nā specimen cervical.No ka maʻi maʻi ʻāʻī o ka ʻōpū, hōʻike ka hopena maikaʻi i ka piʻi ʻana o ka papa 2 a i ʻole ke kula kiʻekiʻe/ʻoi aku ka nui o ka cervical intraepithelial neoplasia (CIN2+, me CIN2, CIN3, adenocarcinoma in situ, a me ka maʻi maʻi cervical), kahi e koi hou ai i ka colposcopy a/a i ʻole ka histopathological nānā. .ʻO ka mea ʻē aʻe, hōʻike nā hopena hōʻike maikaʻi ʻole he haʻahaʻa ka pilikia o CIN2+, akā ʻaʻole hiki ke hoʻopau loa i ka pilikia.Pono e hoʻokumu ʻia ka hōʻailona hope ma ka colposcopy a/a i ʻole nā hopena histopathological.Eia kekahi, no ka ma'i 'a'ai endometrial, hō'ike ka hopena maika'i i ka pi'i 'ana o ka ma'i 'a'ai mua o ka endometrial a me ka ma'i 'a'ai, kahi e pono ai ka nānā 'ana i ka histopathological o ka endometrium.ʻO ka mea ʻē aʻe, hōʻike nā hopena hōʻike maikaʻi ʻole i ka haʻahaʻa o ka hopena o ka endometrial precancerous lesions a me ka maʻi kanesa, akā ʻaʻole hiki ke kāpae ʻia ka pilikia.Pono e hoʻopaʻa ʻia ka ʻike hope loa ma nā hopena hoʻokolokolo histopathological o ka endometrium.
ʻO PCDHGB7 kahi lālā o ka ʻohana protocadherin γ gene cluster.Ua ʻike ʻia ʻo Protocadherin e hoʻoponopono i nā kaʻina biological e like me ka hoʻonui ʻana o ka cell, cell cycle, apoptosis, invasion, migration and autophagy of tumor cells ma o nā ala hōʻailona like ʻole, a me kona silencing gene i kumu ʻia e ka hypermethylation o ka mea hoʻolaha e pili kokoke ana i ka hanana a me ka ulu ʻana. o na ma'i ma'i he nui.Ua hōʻike ʻia e pili ana ka hypermethylation o PCDHGB7 me nā ʻano ʻokoʻa like ʻole, e like me ka lymphoma non-Hodgkin, ka maʻi ʻaʻai umauma, ka maʻi maʻi cervical, ka maʻi maʻi endometrial a me ka maʻi maʻi ʻaʻai.
KUMU HOIKE
Loaʻa i kēia pahu ka mea hoʻoheheʻe nucleic acid a me ka reagent ʻike PCR.Hoʻokuʻu ʻia ka ʻakika nucleic e ka magnetic-bead-based method.Hoʻokumu ʻia kēia pahu ma ke kumu o ka fluorescence quantitative PCR, me ka hoʻohana ʻana i ka methylation-specific real-time PCR reaction no ka nānā ʻana i ka DNA template, a ʻike pū i nā pūnaewele CpG o PCDHGB7 gene a me ka marker control marker internal reference gene fragments G1 a me G2.Ua helu ʻia ka pae methylation o PCDHGB7 i ka laʻana, a i ʻole ka waiwai Me, e like me ka PCDHGB7 gene methylated DNA amplification Ct waiwai a me ka waiwai Ct o ka kuhikuhi.Hoʻoholo ʻia ka PCDHGB7 gene hypermethylation kūlana maikaʻi a maikaʻi ʻole paha e like me ka waiwai Me.
