TAGMe DNA Methylation Detection Kits(qPCR) no ka maʻi maʻi ʻāʻī
NA HI'O HUA
pololei
Hoʻopaʻa ʻia ma luna o 36000 mau lāʻau lapaʻau i loko o nā haʻawina ʻelua makapō multi-center, loaʻa ka huahana i kahi kikoʻī o 94.3% a me kahi sensitivity o 96.0%.
ʻoluʻolu
Hiki ke hoʻopau ʻia ka ʻenehana ʻike ʻike methylation Me-qPCR kumu i hoʻokahi ʻanuʻu i loko o 3 mau hola me ka ʻole o ka hoʻololi bisulfite.
Mamua
Hiki ke hoʻonui ʻia ka nānā ʻana i ka maʻi maʻi maʻi ʻāʻī a hiki i ke kahua o ka pae kiʻekiʻe (precancerous lesions).
ʻOtometi
Hoʻopili ʻia me ka polokalamu loiloi hopena maʻamau, ʻokoʻa ka wehewehe ʻana i nā hopena a hiki ke heluhelu pololei ʻia.
KA MANAO ANA
Hoʻohana ʻia kēia kit no ka ʻike in vitro qualitative o ka hypermethylation o ka gene PCDHGB7 i nā specimen cervical.Hōʻike ka hopena maikaʻi i ka piʻi ʻana o ka papa 2 a i ʻole ke kula kiʻekiʻe a i ʻole nā neoplasia cervical intraepithelial neoplasia (CIN2+, me CIN2, CIN3, adenocarcinoma in situ, a me ka maʻi ʻaʻai cervical), kahi e koi hou ai i ka colposcopy a/a i ʻole ka histopathological nānā.ʻO ka mea ʻē aʻe, hōʻike nā hopena hōʻike maikaʻi ʻole he haʻahaʻa ka pilikia o CIN2+, akā ʻaʻole hiki ke hoʻopau loa i ka pilikia.Pono e hoʻokumu ʻia ka hōʻailona hope ma ka colposcopy a/a i ʻole nā hopena histopathological.ʻO PCDHGB7 kahi lālā o ka ʻohana protocadherin γ gene cluster.Ua ʻike ʻia ʻo Protocadherin e hoʻoponopono i nā kaʻina biological e like me ka hoʻonui ʻana o ka cell, cell cycle, apoptosis, invasion, migration and autophagy of tumor cells ma o nā ala hōʻailona like ʻole, a me kona silencing gene i kumu ʻia e ka hypermethylation o ka mea hoʻolaha e pili kokoke ana i ka hanana a me ka ulu ʻana. o na ma'i ma'i he nui.Ua hōʻike ʻia e pili ana ka hypermethylation o PCDHGB7 me nā ʻano ʻokoʻa like ʻole, e like me ka lymphoma non-Hodgkin, ka maʻi ʻaʻai umauma, ka maʻi maʻi cervical, ka maʻi maʻi endometrial a me ka maʻi maʻi ʻaʻai.
KUMU HOIKE
Loaʻa i kēia pahu ka mea hoʻoheheʻe nucleic acid a me ka reagent ʻike PCR.Hoʻokuʻu ʻia ka ʻakika nucleic e ka magnetic-bead-based method.Hoʻokumu ʻia kēia pahu ma ke kumu o ka fluorescence quantitative PCR, me ka hoʻohana ʻana i ka methylation-specific real-time PCR reaction no ka nānā ʻana i ka DNA template, a ʻike pū i nā pūnaewele CpG o PCDHGB7 gene a me ka marker control marker internal reference gene fragments G1 a me G2.Ua helu ʻia ka pae methylation o PCDHGB7 i ka laʻana, a i ʻole ka waiwai Me, e like me ka PCDHGB7 gene methylated DNA amplification Ct waiwai a me ka waiwai Ct o ka kuhikuhi.Hoʻoholo ʻia ka PCDHGB7 gene hypermethylation kūlana maikaʻi a maikaʻi ʻole paha e like me ka waiwai Me.
Nā hiʻohiʻona noiʻi
ʻO ka nānā mua
Nā kānaka olakino
Ka Loiloi Pilikia
Ka heluna kanaka pilikia nui (maikaʻi no ka maʻi papilloma kanaka kiʻekiʻe (hrHPV) a i ʻole maikaʻi no ka cytology exfoliation cervical)
Nānā Hoʻi hou
Ka heluna kanaka postoperative (me ka mōʻaukala o nā ʻeha cervical kiʻekiʻe a i ʻole ka maʻi maʻi cervical)
ʻO ke koʻikoʻi o ke kino
Ka nānā mua ʻana no ka heluna kanaka olakino:Hiki ke nānā pono ʻia ka maʻi maʻi ʻāʻī a me nā ʻeha precancerous
Ka loiloi pilikia i ka heluna kanaka pilikia nui:Hiki ke hana ʻia ka hoʻokaʻawale ʻana i ka pilikia ma nā heluna kanaka maʻi HPV e alakaʻi i ka ʻike hoʻāʻo ʻana ma hope
Ka nānā hou ʻana no ka heluna postoperative:Hiki ke hana ʻia ka nānā ʻana i ka heluna kanaka ma hope o ka hana ʻana no ka pale ʻana i ka lohi o ka mālama ʻana ma muli o ka hoʻi hou ʻana
ʻOhi laʻana
ʻano laʻana: E kau i ka mea hoʻoheheʻe cervical ma ka cervical os, e hamo mālie i ka pulupulu cervical a hoʻololi i 4-5 mau manawa ma ka ʻākau, wehe mālie i ka pulupulu cervical, hoʻokomo i loko o ka solution mālama cell, a e lepili iā ia no ka nānā ʻana.
Mālama ʻia nā laʻana:Hiki ke mālama ʻia nā laʻana ma ka lumi wela a hiki i 14 lā, ma 2-8 ℃ a hiki i 2 mahina, a ma -20±5 ℃ a hiki i 24 mahina.
Kaʻina ʻike: 3 mau hola (me ka ʻole o ka hana lima)
DNA Methylation Detection Kits (qPCR) no ka Urothelial Cancer
| Hoʻohana lapaʻau | ʻO ka hōʻailona kōkua kino o ka maʻi maʻi cervical |
| ʻAno ʻike | PCDHGB7 |
| ʻAno laʻana | Nā hōʻailona cervical wahine |
| ʻano hoʻāʻo | ʻO ka ʻenehana PCR nui fluorescence |
| Hoʻohālike kūpono | ABI7500 |
| Hoʻopili kikoʻī | 48 hōʻike / pahu |
| Nā Kūlana Waihona | Pono e mālama ʻia ʻo Kit A ma 2-30 ℃ Pono e mālama ʻia ʻo Kit B ma -20±5 ℃ Pono a hiki i 12 mahina |
